The Next-Generation Sequencing Core offers comprehensive
high-throughput sequencing services from experiment
design to basic data analysis to members of the
University of Pennsylvania
Frequently Asked Questions
- Is this the sequencing core I want?
- What does it cost?
- How do I get started?
- Selecting BARCODES
- How do I make *-Seq libraries?
- Does the NGSC make *-Seq libraries?
- When can I bring my samples in?
- How are my samples doing?
- How do I download data?
- Where is my old data?
- Where is the old TessLA browser?
Sample Status
Jonathan's Calendar
seqeuencing-queue from trello
Charges
Updates
Services Offered by the FGC
| Experiment Design | Advice on techniques, sequencing depth, and analysis. |
|---|---|
| Microarrays | Agilent microarrays for RNA expression experiments. |
| Sample Quality Assessments | Agilent bioAnalyzer and TapeStation, *** quBit |
| Library Preparation | Various protocols for Illumina sequencing. |
| Illumina hiSeq sequencing | 50bp and 100bp, single-read and paired-end |
| Illumina miSeq sequencing | 50 to 500bp total, single-read and paired-end |
| Data Analysis | Basic and custom analysis for microarray and sequencing data. |
