Perelman School of Medicine | University of Pennsylvania
| NGSC - FAQs |
Next-Generation Sequencing Core Perelman School of Medicine | University of Pennsylvania |
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The NGSC currently has the following active sequencers
The HiSeq 4000 is used for most sequencing whenever possible since it offers the lowest cost per read.
The NextSeq is most useful when a very short turn around time is needed. It can also be used in self-service mode with training.
The MiSeq is a low output machine used for testing libraries or for modest metagenomics or other targeted sequencing experiments. It can also be used in self-service mode with training.
This table summarizes the expected data volumes and prices for each machine and its possible running modes.
| Service Code | Price | Length | Ends | Reads or Pairs | Data | Cost | Cost | Sequencer | Chemistry |
| [bp] | SR/PE | [millions] | [GB] | [$/GB] | [$/MR] | ||||
| 050PE HiSeq 4000 | $2081.80 | 50 | PE | 325 | 32 | $64 | $6 | HiSeq 4000 | Vn |
| 050SR HiSeq 4000 | $1367.52 | 50 | SR | 325 | 16 | $84 | $4 | HiSeq 2500 | Rapid-V2 |
| 075PE ns500 high | $4810.74 | 75 | PE | 400 | 60 | $80 | $12 | NextSeq 500 | high |
| 075PE ns500 mid | $2588.52 | 75 | PE | 130 | 20 | $133 | $20 | NextSeq 500 | mid |
| 075SR ns500 high | $3076.92 | 75 | SR | 400 | 30 | $103 | $8 | NextSeq 500 | high |
| 100SR HiSeq 4000 | $1746.03 | 100 | SR | 325 | 32 | $54 | $5 | HiSeq 2500 | Rapid-V2 |
| 150PE ns500 high | $7069.59 | 150 | PE | 400 | 120 | $59 | $18 | NextSeq 500 | high |
| 150PE ns500 mid | $3516.48 | 150 | PE | 130 | 39 | $90 | $27 | NextSeq 500 | mid |
| 150PE HiSeq 4000 | $3083.02 | 150 | PE | 325 | 98 | $32 | $9 | HiSeq 4000 | Vn |
| miSeq 300 | $2148.96 | 150 | PE | 20 | 6 | $358 | $107 | MiSeq | V2 |
| miSeq 50 | $1849.81 | 50 | SR | 20 | 1 | $1,850 | $92 | MiSeq | V2 |
| miSeq 500 | $2307.69 | 250 | PE | 20 | 10 | $231 | $115 | MiSeq | V2 |
| miSeq micro 300 | $1373.62 | 150 | PE | 4 | 1 | $1,145 | $343 | MiSeq | V2 |
| miSeq nano 300 | $1190.47 | 150 | PE | 1 | 0 | $3,968 | $1,190 | MiSeq | V2 |
| miSeq nano 500 | $1263.73 | 250 | PE | 1 | 0 | $2,527 | $1,264 | MiSeq | V2 |
| miSeq v3_600 | $2820.51 | 300 | PE | 20 | 12 | $235 | $141 | MiSeq | V3 |